Cell Culture

Cell culture is a fundamental technique in biological research, playing a pivotal role in fields such as cell biology, microbiology, immunology, cancer research, and drug development. It allows researchers to study cells outside of their natural environment under controlled laboratory conditions. From understanding cellular mechanisms to developing therapeutic treatments, cell culture is a versatile tool that forms the backbone of many modern experimental approaches.

This course is designed to provide a comprehensive guide to both the theoretical and practical aspects of cell culture, making it an essential resource for researchers. The content is structured into three main sections: preparation, cell passage, and storage, revival, and shipment. Each section offers detailed protocols, observations, and troubleshooting techniques, ensuring learners gain a thorough understanding of essential processes.

Throughout the course, you will explore the different types of cells used in culture, the specific conditions needed to maintain cell viability, and how to scale up or adjust culture systems depending on experimental requirements. You will also learn critical techniques for maintaining healthy cell lines, including media preparation, cell counting, and identifying and resolving contamination issues.

Beyond basic cell culture methods, this course dives into more advanced topics such as cryopreservation for long-term storage, monoclonal antibody production via hybridoma technology, and the specialized use of cell lines for research on emerging viruses like SARS-CoV-2.

By the end of the course, learners will have developed a solid foundation in the principles and practices of cell culture. Equipped with this knowledge, researchers will be able to implement these techniques in their experiments, contributing to scientific advancements in fields ranging from basic biology to applied biomedical research.

This course, led by Prof. Shingo Inoue, provides both theoretical foundations and practical applications, including hands-on experience with Cell Culture technques. Participants will gain fundamental skills needed for Cell Culture